Biuret reagent B, 500ml

Synonyms: • Protein test solution

CAS Number: 108-19-0

Molecular Weight:

Description:

Is used for detecting the presence of at least two peptide bonds in a molecule. Despite its name, the reagent does not in fact contain biuret [(H2N−CO−)2NH]. The test is named so because it also gives a positive reaction to the peptide-like bonds in the biuret molecule.

In this assay, the copper(II) binds with nitrogen atoms present in the peptides of proteins. In a secondary reaction, the copper(II) is reduced to copper(I). Buffers, such as Tris and ammonia interfere with this assay, therefore rendering this assay inappropriate for protein samples purified from ammonium sulfate precipitation. Due to its insensitivity and little interference by free amino acids, this assay is most useful for whole tissue samples and other sources with high protein concentration

Safety:

Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation.

Eye: Causes severe eye burns. May result in corneal injury. Contact may cause ulceration of the conjunctiva and cornea.

Skin: Causes skin burns. May cause deep, penetrating ulcers of the skin.

Ingestion: Causes gastrointestinal tract burns. Causes severe pain, nausea, vomiting, diarrhea, and shock. Ingestion may produce severe abdominal pain, corrosion of the lips, mouth, tongue, and pharynx, and the vomiting of large pieces of mucosa.

Inhalation: Irritation may lead to chemical pneumonitis and pulmonary edema. Causes severe irritation of upper respiratory tract with coughing, burns, breathing difficulty, and possible coma. Exposure to the mist may cause multiple small burns with temporary loss of hair.

Chronic: Prolonged or repeated skin contact may cause dermatitis.